MersV1, Main, Exploration, bibRecord, 004C77

A study of side reactions occurring during synthesis of oligodeoxynucleotides containing O6-alkyldeoxyguanosine residues at preselected sites.

Identifieur interne : 004C77 ( Main/Exploration ); précédent : 004C76; suivant : 004C78

A study of side reactions occurring during synthesis of oligodeoxynucleotides containing O6-alkyldeoxyguanosine residues at preselected sites.

Auteurs : H. Borowy-Borowski ; R W Chambers

Source :

Biochemistry [ 0006-2960 ] ; 1987.

RBID : pubmed:3607028

Descripteurs français

KwdFr :
- Alkylation, Désoxyguanosine (analogues et dérivés), Indicateurs et réactifs, Oligodésoxyribonucléotides (synthèse chimique), Spectroscopie par résonance magnétique, Séquence nucléotidique.
MESH :
- analogues et dérivés : Désoxyguanosine.
- synthèse chimique : Oligodésoxyribonucléotides.
- Alkylation, Indicateurs et réactifs, Spectroscopie par résonance magnétique, Séquence nucléotidique.

English descriptors

KwdEn :
- Alkylation, Base Sequence, Deoxyguanosine (analogs & derivatives), Indicators and Reagents, Magnetic Resonance Spectroscopy, Oligodeoxyribonucleotides (chemical synthesis).
MESH :
- chemical , analogs & derivatives : Deoxyguanosine.
- chemical , chemical synthesis : Oligodeoxyribonucleotides.
- Alkylation, Base Sequence, Indicators and Reagents, Magnetic Resonance Spectroscopy.

Abstract

As part of our studies on the molecular mechanisms of mutation by carcinogens we have synthesized 12 oligonucleotides (15-mers) containing an O6-alkylguanine residue at a preselected position for use as primers in the enzymatic synthesis of biologically active DNA. Ten of these oligonucleotides are derived from a minus strand sequence carrying the modified nucleotide in the third codon of gene G of bacteriophage phi X174 DNA. Two others are derived from plus strand sequences carrying the modification in the 12th codon of the human Ha-ras protooncogene. During this work several potentially serious side reactions, which could complicate interpretation of mutagenesis data, were observed. This paper describes a detailed study of these reactions. Since we were unable to avoid undesirable side products, we developed simple chromatographic methods for detecting and removing them.

DOI: 10.1021/bi00383a010
PubMed: 3607028

Affiliations:

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en">A study of side reactions occurring during synthesis of oligodeoxynucleotides containing O6-alkyldeoxyguanosine residues at preselected sites.</title>
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<term>Magnetic Resonance Spectroscopy</term>
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<term>Désoxyguanosine (analogues et dérivés)</term>
<term>Indicateurs et réactifs</term>
<term>Oligodésoxyribonucléotides (synthèse chimique)</term>
<term>Spectroscopie par résonance magnétique</term>
<term>Séquence nucléotidique</term>
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<keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Deoxyguanosine</term>
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<term>Indicators and Reagents</term>
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<term>Indicateurs et réactifs</term>
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<term>Séquence nucléotidique</term>
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<front><div type="abstract" xml:lang="en">As part of our studies on the molecular mechanisms of mutation by carcinogens we have synthesized 12 oligonucleotides (15-mers) containing an O6-alkylguanine residue at a preselected position for use as primers in the enzymatic synthesis of biologically active DNA. Ten of these oligonucleotides are derived from a minus strand sequence carrying the modified nucleotide in the third codon of gene G of bacteriophage phi X174 DNA. Two others are derived from plus strand sequences carrying the modification in the 12th codon of the human Ha-ras protooncogene. During this work several potentially serious side reactions, which could complicate interpretation of mutagenesis data, were observed. This paper describes a detailed study of these reactions. Since we were unable to avoid undesirable side products, we developed simple chromatographic methods for detecting and removing them.</div>
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Serveur d'exploration MERS

A study of side reactions occurring during synthesis of oligodeoxynucleotides containing O6-alkyldeoxyguanosine residues at preselected sites.

A study of side reactions occurring during synthesis of oligodeoxynucleotides containing O6-alkyldeoxyguanosine residues at preselected sites.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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